Nov 30, 2007 · Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.Cited by: 17005. Aug 25, 2006 · Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable.Cited by: 22034.
Nov 30, 2007 · These results are proof of principle that pluripotent stem cells can be generated from somatic cells by the combination of a small number of factors. In the current study, we sought to generate iPS cells from adult human somatic cells by optimizing retroviral transduction in human fibroblasts and subsequent culture conditions.Cited by: 17005. Nov 30, 2007 · Summary. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.Cited by: 17005.
1. Induction of iPS Cells from Adult HDF 2. Expression of hES Cell-Marker Genes in Human iPS Cells 3. Analyses Promoter Regions of Development-Associated Genes in Human iPS Cells 4. High Levels of Telomerase Activity and Exponential Proliferation of Human iPS Cells 5. Embryoid Body-Mediated Differentiation of Human iPS Cells 6. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichisaka T., Tomoda K., Yamanaka S. Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells.
Induced pluripotent stem cells (iPSCs) can be produced from various somatic cells and have the ability to differentiate into various cells and tissues of the body. Jul 22, 2010 · Generation of pluripotent stem cells from adult fibroblasts. We cultured primary cardiac fibroblasts (cFBs) from an adult C57/BL mouse. To exclude potential resident stem cells (eg, adult cardiac stem cells), we collected c-kit− cells and cultured them to at least passage 4 .
Aug 25, 2006 · Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development.Cited by: 22034. Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from adult cells. The iPSC technology was pioneered by Shinya Yamanaka ’s lab in Kyoto, Japan, who showed in 2006 that the introduction of four specific genes encoding transcription factors could convert adult cells into pluripotent stem cells. .